Fig. 7: Analysis of XIST RNA coating during differentiation of female hESCs.
From: Preventing erosion of X-chromosome inactivation in human embryonic stem cells
a Schematic of hESC differentiation into embryoid bodies (EBs) with three different media formulations: a commercially available AggreWellTM medium; XF medium lacking bFGF; and XF medium lacking bFGF but containing 0.98 mM LiCl. b Percentage of nuclei with XIST RNA coating in EBs generated from hESC lines UM77-2 and UM63-1. EBs generated and cultured in XF medium with LiCl and AggreWellTM medium lost a significant proportion of XIST RNA coating per colony compared to EBs generated and cultured in XF medium (general linear model comparison; p < 0.001). At least 100 nuclei were counted per colony for hESC RNA FISH quantification. The total number of colonies quantified at each passage range between 10 and 17 and are cataloged in source data. Source data are provided as a Source Data file.
