Fig. 2: HSC niches are formed in mice lacking Runx1 or Runx2.
From: Runx1 and Runx2 inhibit fibrotic conversion of cellular niches for hematopoietic stem cells
a–d Bone marrow from 3-week-old control, Prx1-Cre;Runx1f/f (a) or Prx1-Cre;Runx2f/f;CXCL12-GFP mice (b, c, d) was analyzed. a Total hematopoietic cell counts and the numbers of LT-HSCs, MEPs, proerythroblasts (pro-E), GMPs, CLPs, pro-B cells, and pre-B cells (n = 4 mice per group). b von Kossa and toluidine blue staining of femurs. Bars: 1 mm. c Confocal microscopy images showing expression of CXCL12-GFP. Bars: 100 μm. d Frequencies of LT-HSCs, MEPs, pro-E, GMPs, CLPs, pro-B cells, and pre-B cells (n = 4 mice per group). All error bars represent SD of the mean. Statistical significances were calculated using the two-tailed unpaired Student’s t-test. Source data are provided as a Source Data file.
