Fig. 3: Markedly reduced HSPCs and impaired HSC niches in mice lacking both Runx1 and Runx2 in CAR cells.
From: Runx1 and Runx2 inhibit fibrotic conversion of cellular niches for hematopoietic stem cells
a–h Femurs and tibias (a–c, f–h), spleen (d), and peripheral blood (e) from 20- to 24-week-old tamoxifen-treated control and Ebf3-CreERT2;Runx1f/fRunx2f/f mice were analyzed. a Total hematopoietic cell counts, frequencies of LT-HSCs, and the numbers of LT-HSCs, MEPs, pro-E, MkPs, GMPs, Gr-1hi granulocytes, CLPs, pro-B cells, pre-B cells, mature B cells, pDCs, and NK cells (n = 10 mice per group). b The numbers of functional HSCs were estimated using repopulating units (RUs) (n = 5 mice per group). c The numbers of megakaryocytes per high powered field from bone marrow (n = 6 mice per group). d The numbers of LT-HSCs, MEPs, and GMPs in spleen (n = 10 mice per group). e Platelet counts in peripheral blood (PB) (n = 10 mice per group). f Cell surface expression of Sca-1 in CD31-CD45-Ter119-PDGFRβ+ CAR cells. g, h Relative mRNA expression levels of Osx, PDGFRβ, LepR, CXCL12, SCF, IL-7, Foxc1, Ebf1, Ebf3 (g), Col1a1, Col3a1, Col6a3, Gli1, PDPN, and PDGFRα (h) in sorted CAR cells (n = 10 mice per group). All error bars represent SD of the mean. Statistical significances were calculated using the two-tailed unpaired Student’s t-test. Source data are provided as a Source Data file.
