Fig. 5: S. aureus adherence to healthy human corneocytes is mediated by FnBPB. | Nature Communications

Fig. 5: S. aureus adherence to healthy human corneocytes is mediated by FnBPB.

From: Fibronectin binding protein B binds to loricrin and promotes corneocyte adhesion by Staphylococcus aureus

Fig. 5

SH1000 deficient in ClfA, ClfB, FnBPA, and FnBPB [SH1000 4X] carrying pALC2073::fnbB (pFnBPB) and pALC2073 were grown to exponential phase (OD600 = 0.35), incubated with healthy human corneocytes at 37 °C, washed, and adherent bacteria were stained with crystal violet. Corneocytes were visualised using light microscopy and the number of bacteria adhering to ten corneocytes was counted. Each datum point represents the mean number of bacteria per corneocyte. The bars represent the means of four independent experiments, with error bars showing the standard deviation. A no bacteria control was used where PBS alone was incubated with the corneocytes. Statistical analysis was performed using a one-way ANOVA with a Dunnett´s multiple comparison test to compare variances between S. aureus SH1000 4X (pFnBPB), S. aureus SH1000 4X (pALC2073), and the no bacteria control. *SH1000 4X (pFnBPB) vs SH1000 4X (pALC2073), P = 0.0186; SH1000 4X (pFnBPB) vs 1X PBS, P = 0.0139.

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