Fig. 4: In vitro characterization of non-enzymatic and enzymatic norbornene formation in sordaricin biosynthesis. | Nature Communications

Fig. 4: In vitro characterization of non-enzymatic and enzymatic norbornene formation in sordaricin biosynthesis.

From: Discovery and characterization of a terpene biosynthetic pathway featuring a norbornene-forming Diels-Alderase

Fig. 4

a Overall synthetic scheme for compound 7 and 10. b Cyclization of compound 10 in the absence (left) and presence (right) of SdnG in a 50 mM HEPES buffer, pH 7.4. Decrease at A286 nm (disappearance of the cyclopentadiene chromophore) was used to monitor the cyclization. c First order kinetics of non-enzymatic cyclization of compounds 10 and 7 (inset). The data points and error bars represent the mean and standard deviation of n = 3 independent measures respectively. Errors associated with the kinetic parameters were obtained from fitting. Source data are provided as a Source Data file. d Michalis-Menten kinetics of SdnG with compound 10 as substrate. The data points and error bars represent the mean and standard deviation of n = 3 independent measures respectively. Errors associated with the kinetic parameters were obtained from fitting. Source data are provided as a Source Data file. e DFT calculations of IMDA transition states in water for dialdehyde 7 (TS-1), and carboxylate-aldehyde 10 (TS-2), and aldehyde-alcohol 5 (TS-3).

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