Fig. 2: Agonistic effects of OEA on the HIF-3α-ARNT heterodimer.
From: Identification of oleoylethanolamide as an endogenous ligand for HIF-3α
a Chemical structure of OEA. b Protein thermal shift assays measuring the change in melting temperatures (ΔTm) of HIF-1α-ARNT, HIF-2α-ARNT and HIF-3α-ARNT (i.e., HIF-1, HIF-2 and HIF-3) complex proteins in the presence of 20 μM OEA. c Binding of OEA to the HIF-3α PAS-B domain (KD about 14.0 μM) as measured by SPR. d Effects of OEA on the heterodimerization of HIF-3α-ARNT and HIF-2α-ARNT, detected by TR-FRET. e The effects of HIF-3α overexpression and 25 μM OEA treatment on the expression of HSPA6 in HEK293 cells. **p = 0.0019 for pCMV-Tag4 vs. HIF-3α, *p = 0.0437 for HIF-3α vs. HIF-3α + OEA. Error bars, mean ± SD.; n = 3 (biological replicates) for (d,e). Statistical significance: *p < 0.05, **p < 0.01 (Unpaired two-tailed t test).
