Fig. 2: 1,10-pt treatment mostly leads to a significant decrease in Epl1 promoter binding except for a small group of genes displaying a dramatic Epl1 binding increase.

A Scatter plots comparing Epl1 ChIP-seq signal before and after 1,10-pt treatment at promoters of all 562 genes (gray dots) selected by Martin et al.¹ to study Epl1 promoter binding. Indicated gene categories (RP genes, n = 98; Hsf1 target genes, n = 34) are color-coded on the scatter plots; raw data are from replicate 1 from ref. ¹. B Average Epl1 or Epl1(1-485) (dotted line) binding profiles on genes selected by Martin et al.¹) before (blue curves) or after 15 min of 1,10-pt treatment (red curves). The first four panels show data for all 562 genes divided into four separate categories, as marked: Hsf1 target genes (Hsf1, n = 34), ribosomal protein genes (RP, n = 98), genes where Epl1 ChIP-seq signal increases > 1,2 fold (Increase, n = 123), and all other genes in this group (Other, n = 307). The right-most panel shows the average for all 562 genes. Data are taken from Martin et al.¹ and all plots are centered on the Epl1 signal peak. C Boxplots of log10 EpL1 ChIP-seq signal at promoters of the four gene categories defined in B before (−) or after (+) 15 min of 1,10-pt treatment. The ChIP-seq signal is quantified in a region delimited as the 100 bp region upstream and downstream of the Epl1 signal peak defined by Martin et al.¹. Asterisks show significant difference according to the Wilcoxon test (*P < 0.05, **P < 0.01, ***P < 0.001, ns not significant). D Most significant motifs found in genes at which Epl1 binding increases (>1,2-fold) or decreases (<0,8-fold) after 1,10-pt treatment. E values and the TF associated with the consensus binding motif identified by MEME are indicated. E Genome browser tracks showing Epl1, Rap1 and Tbf1 ChIP-seq read counts at the promoter regions (roughly demarcated by dotted lines) of the indicated genes before (t = 0) or 15 min after 1,10-pt treatment. Raw data are from Martin et al.¹, Knight et al.¹⁷, and Preti et al.¹⁸ and for Epl1, Rap1, and Tbf1 ChIP-seq, respectively.