Fig. 6: Mitochondrial 1C metabolism supports cell motility and depends on mitochondrial THF availability.

a Migration of MDA-MB-468 cells upon 2.5 mM Metformin and 50 nM MTX and respective AUC; mean ± SEM of independent experiments (n = 3); Brown-Forsythe and Welch one-way ANOVA with Dunnett’s multiple comparisons test. b Migration of MDA-MB-468 cells upon MTHFD2 knockdown and 50 nM MTX and respective AUC; mean ± SEM of independent experiments (n = 4); Brown-Forsythe and Welch one-way ANOVA with Dunnett’s multiple comparisons test. c Migration of 4T1 cells upon Mthfd2 knockdown and 75 nM MTX and respective AUC; mean ± SEM of independent experiments (n = 3); Brown-Forsythe and Welch one-way ANOVA with Dunnett’s multiple comparisons test. d Migration of 4T1 cells upon Mthfd1l knockdown and 75 nM MTX and respective AUC; mean ± SEM of independent experiments (n = 3); Brown-Forsythe and Welch one-way ANOVA with Dunnett’s multiple comparisons test. e Migration of MDA-MB-468 cells upon MTHFD1L knockout and 50 nM MTX and respective AUC; mean ± SEM of independent experiments (n = 3); Brown-Forsythe and Welch one-way ANOVA with Dunnett’s multiple comparisons test. f ALDH1L2 and MTHFD1L protein expression in MDA-MB-468 and 4T1 cells. g Signal intensity of ALDH1L2 on Western Blot was quantified relative to β-actin as loading control. Mean ± SEM (n = 3); unpaired t-test with Welch’s correction. h MTHFD1L- and ALDH1L2-dependent reactions in mitochondrial 1C metabolism. i Migration of MDA-MB-468 cells upon MTHFD1L knockout, ALDH1L2 knockdown and 50 nM MTX and respective AUC; mean ± SEM of independent experiments (n = 3); Brown-Forsythe and Welch one-way ANOVA with Dunnett’s multiple comparisons test. j Model summarizing the autarkic activity of mitochondrial and cytosolic 1C metabolism and their respective relevance for cell migration and cell proliferation. Source data are provided as a Source Data file.