Fig. 5: HigBTAC interactions with the ribosome and with cspA mRNA. | Nature Communications

Fig. 5: HigBTAC interactions with the ribosome and with cspA mRNA.

From: Substrate recognition and cryo-EM structure of the ribosome-bound TAC toxin of Mycobacterium tuberculosis

Fig. 5

Interaction between HigBTAC [K95A] and: a helix 18 of 16S rRNA; b the region delimited by helices 30 and 31 of 16S rRNA; c helix 34 of 16S rRNA; and d helix 44 of 16S rRNA and helix 69 of 23S mRNA. e Interactions between the HigBTAC [K95A] catalytic site and the CCA codon of the cspA mRNA. In all figures, HigBTAC [K95A] is red, the cspA mRNA is purple, the 16S rRNA is light yellow, and the 23S rRNA is light blue. Residues and nucleotides within 4 Å of each other are indicated. f M. smegmatis transformed with pGMC-vector (−), HigBTAC wild-type or its mutant derivatives were serial diluted, spotted on LB streptomycin agar plates with or without anhydrotetracycline (Atc ng/mL) inducer and incubated 3 days at 37 °C. Representative results of three independent experiments are shown.

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