Fig. 5: MUFA-containing PI species indicate stress in mice with Scd1 defect.

Liver, white abdominal fat, hind leg skeletal muscle, and skin were obtained from wildtype mice (wt) and mice homozygous for the Scd1^ab-2J allele (Scd1^ab-2J). a Protein expression of BiP and p38 MAPK, p38 MAPK phosphorylation, and the ratio of LC3BII/LC3BI protein levels in liver. b Hepatic proportion of MUFAs in PI and PC; MUFAs: 16:1, 18:1 (LTR P = 0.00000008, 0.00000003). c Heatmap showing the changes in the PI profile of liver, fat, skeletal muscle, and skin from Scd1^ab-2J mice as compared to wt mice. Data are given as percentage of the relative PI abundance in wt tissues. d Hepatic proportion of PI(18:1/18:1) and PI(16:1/18:1) (LTR P = 0.00001, 0.00003). e Volcano plots highlighting PI and PC species that are regulated in murine tissues from Scd1^ab-2J mice relative to wt mice. Comparisons show the mean difference of percentage changes and the negative log10(adjusted P value). Adjusted P values given vs. vehicle control; two-tailed multiple unpaired student t tests with correction for multiple comparisons using a two-stage linear step-up procedure by Benjamini, Krieger, and Yekutieli (false discovery rate 5%). Phospholipids containing two MUFAs (“MUFA/MUFA”) or one MUFA in combination with SFA or PUFA (“MUFA”) are indicated by color. Mean (c, e) or mean + s.e.m. and single data (a, b, d) from n = 6 (wt) and n = 5 (Scd1^ab-2J; a for BiP, wt) mice. P values as indicated; two-tailed unpaired student t test.