Fig. 7: PI(18:1/18:1) pulses shape the cellular stress response.
From: PI(18:1/18:1) is a SCD1-derived lipokine that limits stress signaling
a Cellular content of PI(18:1/18:1) directly after addition of the fusogenic phospholipid mixture with 3 nmol PI(18:1/18:1) to fibroblasts. b Time-dependent decrease of excess PI(18:1/18:1). c–i Fibroblasts were treated with vehicle or CAY10566 (CAY, 3 µM) for 48 h, and PI(18:1/18:1) (3 nmol) was incorporated into cells using fusogenic liposomes 8 h before harvesting. c Time-scale. d Phosphorylation of p38 MAPK. e Ratio of LC3BII/LC3BI protein levels. f PARP cleavage; cl., cleaved. g Fibroblast morphology; scale bar, 100 µm. Phase contrast images are representative of three independent experiments. h Protein expression of BiP. i Cell numbers. Western blots are representative of three (d, e) or four (f, h) independent experiments. Blots shown in panel d and f and e and h derive from the same membrane, respectively. Mean + s.e.m. and single data from n = 1 (b), n = 3 (a, d, e, i), n = 4 (f, h) independent experiments. Two-tailed paired student t test (a, i) of log data (d–f, h).
