Fig. 9: PI(18:1/18:1) in systems with varying stress tolerance.

a, c–e, g-j Cellular proportion of PI(18:1/18:1). a IgM^- and IgM⁺ B-cells and B-cell lymphoma from Eµ-Myc-transgenic mice. b Expression, phosphorylation, and substrate turnover of the autophagy-regulatory proteins Ulk1, S6-kinase, and 4E-BP1. Western blots are representative of two independent experiments; * non-specific bands. Each line corresponds to an individual animal. c Wild-type (‘wt’) and sorafenib-resistant (‘resistant’) Huh-7 hepatocarcinoma cells. d Resident peritoneal macrophages from young (6-8 months) and old mice (26 months). e Hematopoietic stem cells (KSLs, upper panel) and myeloid progenitor cells (MPs, lower panel) from young and aged mice. f Cellular proportion of PI(16:1/18:1) in brain from young (6-8 month) and old male and female mice (26 month). g, h Influence of starvation on planarians with impaired ER stress adaption. g Lipids were extracted from either 48 h (left panels) or 4 days (right panels) regenerating planarians. gfp dsRNA (gfp(RNAi)) was injected as control. h Relative expression in TPMs of differentially regulated genes related to p38 MAPK activation and stress signaling based on the transcriptome analysis of starved, gfp or cct3A RNAi-treated planarians at 72 h of regeneration. Significance was determined by q-value (false discovery rate (FDR)) < 0.1 for pairwise comparisons. i, j Mice were inoculated with P. gingivalis and housed for 16 weeks on normal, 16:0-enriched, or 18:1-enriched diet or first for eight weeks on 16:0-enriched and then eight weeks on 18:1-enriched diet. Mean + s.e.m. and single data from n = 2 (e, lower panel), n = 3 (e, upper panel, g, h), n = 4 (a for control, f for old female), n = 5 (a for IgM⁺ tumor, c, d, f, i, normal diet for non-infected mice and 16:0/18:1-treated non-infected mice, j, normal diet for non-infected mice), n = 6 (a for IgM^- tumor, i, j). P values as indicated; two-tailed unpaired student t test (c, d, e, g) of log data (a, f, i, j).