Fig. 5: Distances between codon-specific Ramachandran plots are related to parameters of the translation process.
Left: The mean absolute difference in the relative translation speed as a function of the mean distance between backbone dihedral angle distributions for pairs of codons translated unambiguously by the same single tRNA. The two quantities are positively correlated (r2 = 0.6). Translation speed data and confidence intervals are reproduced from Chevance et al. (2014). Translation speed (vertical) error bars were calculated from Fig. S1 A and B in Chevance et al. (2014) based on 3 or more independent assays; distribution distance (horizontal) error bars were obtained from 250 bootstrap samples. Both error bars indicate 1σ. Right: Pairwise distances between backbone dihedral angle distributions of codons translated unambiguously by the same tRNA (green) or two distinct tRNAs (red), sorted in ascending order (left) and as cumulative histograms (right). Noncognate codon pairs tend to exhibit a significantly bigger distance. Horizontal lines indicate means. In both plots, the normalized L1 distances are reported with the ±σ confidence intervals calculated on 1000 bootstraps.
