Fig. 2: TF clustering propensity modulates gene expression amplification.
From: Modulating gene regulation function by chemically controlled transcription factor clustering
a Schematic of the experiment. b Flow cytometry quantifications of the reporter response under various TF clustering propensity conditions for five separate synthetic systems. In all conditions, iRFP fluorescence levels were quantified 72 h post the addition of doxycycline (0.5 μg/ml for U2OS cells and 0.1 μg/ml for CHO cells) and indicated rapamycin concentration. Fold change was calculated by normalizing to zero rapamycin condition. U2OS-7TetO: Monoclonal U2OS cells containing the system depicted in Fig. 1a; U2OS-1TetO: Analogous to U2OS-7TetO except that the reporter gene contains only 1x TetO site; U2OS-w/oTAD: Polyclonal U2OS cells containing the system depicted in Fig. 1a except that the rTetR is not fused to a trans-activation domain; CHO-Gal4: Monoclonal CHO cells containing the system depicted in Supplementary Fig. 1e; CHO-w/oTAD: Polyclonal CHO cells containing the system depicted in Supplementary Fig. 1e except that the Gal4 is not fused to a trans-activation domain. Data are presented as mean ± S.D. (n = 5 biological replicates). p values were calculated by one-way ANOVA and TukeyHSD. Source data are provided, which contain details of statistical tests.
