Fig. 2: REST and ASCL1 control largely non-overlapping programs in SCLC cells.
From: A conserved YAP/Notch/REST network controls the neuroendocrine cell fate in the lungs
a Experimental workflow to identify REST and ASCL1 candidate target genes. GFPhigh cells are non-NE SCLC cells from TKO;Hes1GFP/+ tumors while GFPneg cells represent NE SCLC cells. The mouse SCLC cell line used for the RNA-seq of REST overexpression (OE) is the neuroendocrine KP1 cell line. b Expression of selected neuroendocrine and neuronal-related genes from RNA-seq data of 5-day REST overexpression (n = 3). Error bars, log2 fold change estimate ± SE. c ChIP-seq data showing REST and ASCL1 binding near/within their respective target genes in (b). d Gene ontology (GO) analysis of candidate REST and ASCL1 targets. Note the similar GO terms between the two factors. e Examples of REST, ASCL1 and shared target genes in different gene categories. See also Supplementary Figs. 3 and 4 and Supplementary Data 3–9.
