Fig. 7: YAP1 is required for the transdifferentiation of pulmonary neuroendocrine cells after naphthalene injury.

a Representative immunofluorescence images for Tomato expression (red) and either CC10 or YAP1 (cyan) on consecutive sections from a Ascl1^CreER/+;Rosa26^{LSL-Tom/LSL-Tom};Yap1^+/+ or Yap1^fl/fl mouse 3 weeks after naphthalene-induced lung injury and tamoxifen injection (as in Fig. 4b). DAPI stains DNA in blue in the images on the right. Scale bar, 50 μm. b Quantification (%) of clusters that are Tom⁺ or Tom⁺, CC10⁺ after injury as in (a) (n = 27 clusters for Yap1^+/+ and 32 clusters for Yap1^fl/fl counted from 3 mice per genotype). Fisher’s exact test, two-tailed. c Whole mount fluorescence images of left lung lobes 3 weeks after injury. Images representative of n = 2 independent experiments. Scale bar, 5 mm. d Scatterplot of normal NEBs and PNEC outgrowths after injury as in (c) with each column in the scatterplot representing a single mouse and each data point a tomato-positive cluster in that mouse (n = 2 mice for corn oil controls, 4 mice for naphthalene-treated Yap1^+/+ and 8 mice for naphthalene-treated Yap1^fl/fl). Unpaired t-test was conducted on the number of clusters from each mouse in naphthalene-treated groups that are above the threshold size of the non-injured controls (n = 4 mice for Yap1^+/+, n = 8 mice for Yap1^fl/fl), data represented as mean ± s.d. See also Supplementary Fig. 10. Source data are provided as a Source Data file.