Fig. 3: Inhibition of peptidyl transfer by PR20 and GR20.
From: Ribosome inhibition by C9ORF72-ALS/FTD-associated poly-PR and poly-GR proteins revealed by cryo-EM
a Time progress curves of inhibition of puromycin peptide bond formation by different concentrations of PR20 on E. coli 70S ribosomes (n = 3 independent experiments). Count per minute (cpm). b Dependence of inhibition of peptide bond formation on the lengths of poly-PR and poly-GR repeats (puromycin assay on E. coli ribosomes; apparent inhibition constants are shown on the y axis; n = 2 independent experiments, error bars are standard error). Ki,app was >2000 nM (the highest concentration tested) for both PR4 and GR4 and is shown as an asterisk. c Time progress curves of inhibition of puromycin peptide bond formation by PR20 on rabbit 80S ribosomes (n = 2 independent experiments). Source data are provided as a Source Data file.
