Fig. 5: Cryo-EM shows that PR20 occupies the polypeptide tunnel of the bacterial 70S ribosome.
From: Ribosome inhibition by C9ORF72-ALS/FTD-associated poly-PR and poly-GR proteins revealed by cryo-EM
a Overview of the structure of E. coli 70S•PR20. b PR20 binds to the 70S polypeptide exit channel. c PR20 overlaps with the macrolide antibiotic binding site in the polypeptide tunnel. Erythromycin (Ery; orange) from the E. coli 70S•Ery crystal structure is shown (PDB:4V7U87). d Preincubation of ribosomes with erythromycin (Ery) relieves the inhibition of peptide bond formation by 2 μM PR20, consistent with an overlapping binding site for Ery and PR20. n = 2 independent experiments. Source data are provided as a Source Data file.
