Fig. 2: P1075-binding site in SUR2B.
From: Structural identification of vasodilator binding sites on the SUR2 subunit
a, b Close-up views of the P1075-binding site. TMD1 and TMD2 are colored in pink and blue, respectively. P1075 (yellow) and residues that interact with P1075 are shown as sticks. Putative H-bonds are shown as dashed lines. c Cartoon representation of the interaction between P1075 and SUR2B. The key residues on TMD1 and TMD2 are shown as pink and blue ovals, respectively. d The dose–response activation curves of SUR2B-Kir6.2 KATP channel by P1075 measured by Rb+ efflux assay. Curves were fitted to the Hill equation. Data are presented as mean values ± SD The numbers of independent experiments of WT, H576A, D1008A, I1004L, and T1253M are 3, 3, 3, 4, and 3, respectively. e Effects of metabolism inhibitors (MI, 1 mM 2-deoxy-D-glucose, and 3 μM oligomycin) on KATP constructs containing various SUR2B mutants. Data are presented as mean values ± SD. The numbers of independent experiments of WT, H576A, D1008A, I1004L, and T1253M are 8, 6, 7, 7, and 6, respectively. Source data are provided as a Source Data file.
