Fig. 4: Myo19 maintains mitochondria membrane potential and affects OXPHOS.
a Left: representative thermographs of MDA-MB-231 WT and Myo19 KO cells stained with 100 nM TMRE for 20 min. Scare bar: 20 μm. Right: quantification of TMRE fluorescence intensity per cell. Data are shown as mean ± SD. NWT = 22, NMyo19 KO = 28. ****P < 0.0001. Significance (P-value) was evaluated by two-sided t-test. Source data are provided as a Source Data file. b Flow cytometry analysis of WT and Myo19 KO cells stained with 100 nM TMRM for 20 min. 5 μM FCCP treatment for 10 min was used as positive control. Gating/sorting strategy was performed according to product manual. c Quantification of relative ATP abundance in WT and Myo19 KO cells. Data are shown as mean ± SD. NWT = 3, NMyo19 KO = 3 independent samples. **P = 0.0017. Significance (P-value) was evaluated by two-sided t-test. Source data are provided as a Source Data file. d The representative curves of extracellular acidification rate (ECAR) in MDA-MB-231 scramble and Myo19 knock down (Myo19 KD) cells. Glucose:4.5 g/L; Oligomycin: 1 μM; 2-DG: 50 mM. Data are shown as mean ± SD. Nscramble = 11, NMyo19 KD = 9 independent samples. Source data are provided as a Source Data file. e Quantification of basal and maximal ECAR. Data are shown as mean ± SD. Nscramble = 11, NMyo19 KD = 9 independent samples. ****P < 0.0001. ns, no significance. Significance (P-value) was evaluated by two-sided t-test. Source data are provided as a Source Data file. f The representative curves of oxygen consumption rate (OCR) in scramble and Myo19 KD cells. Oligomycin: 1 μM; FCCP:1 μM; Rotenone: 1 μM. Data are shown as mean ± SD. Nscramble = 10, NMyo19 KD = 10 independent samples. Source data are provided as a Source Data file. g Quantification of basal and maximal OCR. ns, no significance. Data are shown as mean ± SD. Nscramble = 10, NMyo19 KD = 10 independent samples. ****P < 0.0001. Significance (P-value) was evaluated by two-sided t-test. Source data are provided as a Source Data file. h Growth curves of scramble and Myo19 KD cells grown in medium supplemented with the indicated monosaccharides. Left: glucose-based medium. Data are shown as mean ± SD. Nscramble = 5, NMyo19 KD = 5 independent samples. *P = 0.0449. Right: galactose-based medium. Data are shown as mean ± SD. Nscramble = 5, NMyo19 KD = 5 independent samples. **P = 0.0011. Significance (P-value) was evaluated by two-sided t-test. Source data are provided as a Source Data file.
