Fig. 2: Cul5^fl/flCD4-Cre mice develop an unprovoked Th2-mediated lung inflammation.

8–10 or 30–36 week old control and Cul5^fl/flCD4-Cre mice were analyzed. Hematoxylin and Eosin (H&E) and Periodic Acid Schiff (PAS) stain of lung sections. Representative image of stains of lung sections from 8–10-week-old (a) and 30–36 week old (b) mice. Bar graph showing the number of nuclei (c) enumerated by Aperio ImageScope software in 100 mm² area of lungs. PAS score (d) of the lung sections. For a and c, n = 5 for 8–10 week old control and Cul5^fl/flCD4-Cre mice; for 30–36 week old n = 6 control; 7 Cul5^fl/flCD4-Cre mice. For b and d n = 7 control and Cul5^fl/flCD4-Cre mice examined over two independent experiments; Scale bars, 200 μM, zoomed image of PAS is shown in right. e, f Representative flow plots showing the percentages of IL-5⁺ and IL-13⁺ cells among CD44⁺CD4⁺ T cells from lung. g Compiled data showing the frequencies of IL5⁺ cells in 8–10 week old mice n = 9 control; n = 13 Cul5^fl/flCD4- Cre examined over four independent experiments, and 30–36 week old mice n = 10 control; n = 11 Cul5^fl/flCD4-Cre examined in 3 independent experiments. h Numbers of IL-5⁺ cells in 8–10-week old mice n = 5 control; n = 8 Cul5^fl/flCD4- Cre, examined in three independent experiments, and 30–36-week-old mice, n = 8 control; n = 9 Cul5^fl/flCD4-Cre, examined in two independent experiments. i Frequencies and j number of IL-13⁺ cells in lungs. For 8–10 week old mice n = 6 control, n = 8 Cul5^fl/flCD4-Cre, examined in 3 independent experiments; for 30–36 week old mice n = 7 control, n = 6 Cul5^fl/flCD4-Cre, examined in two independent experiments. k Frequencies of eosinophils in the lungs. For 8–10- week old mice, n = 7 control, 8 Cul5^fl/flCD4-Cre examined in three independent experiments, and for 30–36-week-old, n = 6 mice examined in two independent experiments. k Number of eosinophils in the lungs of 8–10- week old mice, n = 7 control, n = 9 Cul5^fl/flCD4-Cre, examined in three independent experiments, and for 30–36-week-old, n = 6 control and Cul5^fl/flCD4-Cre mice examined in two independent experiments. m Serum IgE levels. For 8–10 week old mice, n = 9 control and Cul5^fl/flCD4-Cre mice examined in three independent experiments, and for 30–36-week-old mice, n = 13 control, n = 14 Cul5^fl/flCD4-Cre, examined over 4 independent experiments. Serum IgG1 levels. For 8–10 week old, n = 12 control and Cul5^fl/flCD4-Cre mice examined in three independent experiments, and for 30–36-week-old mice, n = 10 control, n = 11 Cul5^fl/flCD4-Cre, examined over three independent experiments. Data is presented as Mean ± SEM in panels c, d, g–n. p-value is calculated by unpaired two-tailed t-test. AW Airways; BV-blood vessel. Source data are provided as a Source Data file.