Fig. 5: Single-cell transcriptomics reveal differential changes in genes related to RNA splicing and cell cycle upon 5HTP treatment.
From: Serotonin limits generation of chromaffin cells during adrenal organ development
a Adrenal glands (AG) and Organ of Zuckerkandl (ZO) were dissected from E13.5 Wnt1-Cre+/−;R26RTomato+/wt embryos; the tissue was dissociated, and Wnt1Tomato+ cells were sorted into 384-well plates for Smart-seq2 sequencing. Note the absence of statistically-tested compositional effects (see the “Methods” section) between 5HTP-treated and control conditions, shown as UMAPs (bottom panels). b Joint UMAP embedding of cells from AG and ZO from both treated and control groups. c Main marker genes defining each cluster. d Differential gene expression per cluster in 5HTP-treated vs control groups. e Statistically significant differential spliced genes in 5HTP-treated vs control groups. Note that at least four long non-coding RNAs responsible for regulation of Hand2 activity appeared differentially spliced (Uph, Uph.AS2, Uph.AS3, Uph.AS4) and well as Cenpa-related long non-coding RNA. The bottom: UMAP plots showing meaningful differentially spliced and differentially expressed genes.
