Fig. 1: Altered transcriptome of hematopoietic stem and progenitor cells during aging.
From: Epigenetic traits inscribed in chromatin accessibility in aged hematopoietic stem cells
a Experimental strategy. Bone marrow cells from 10-week-old mice (Young) and 20-month-old mice (Aged) were pooled and sorted in duplicate. b PCA plot based on the z-scores of expression values (DESeq2 normalized counts) in each fraction. Blue and red circles indicate young and aged cells (n = 2). c Heatmaps showing the z-scores of expression values (DESeq2 normalized counts) in all DEGs (7711 genes, left panel) and DEGs in HSCs (291 Aged HSC up DEGs and 127 Aged HSC down DEGs, right panel). Genes are divided into five clusters [HSC (purple), HSC/MPP (green), GMP (blue), MEP (orange), and CLP (yellow)-type] based on K-means clustering. d The numbers of up and down DEGs in aged cells in each fraction. e Heatmap showing the z-scores of expression values (DESeq2 normalized counts) of representative DEGs in aged HSCs. f Venn diagram displaying the numbers of common DEGs in aged HSCs between this study and reported studies28,29. P values by Fisher’s exact test are indicated. g The numbers of DEGs common among MPP fractions. Venn diagrams (upper panels). Pyramid plots (lower panels), in which genes at the top and bottom were shared by 4 MPP types and unique to one of the 4 MPP types, respectively. Source data are provided as a Source Data file.
