Fig. 7: Altered histone modifications at DARs in aged HSCs.

a Heatmap showing the levels of H3K4me1, H3K27ac, H3K4me3, and H3K27me3 signals at the range of the center of the Aged HSC open DARs ± 3.0 kb in aged HSCs. Aged HSC open DARs were subdivided into promoters and enhancers (active, primed, and inactive enhancers) based on their location and histone modification profiles. The number of DARs are indicated in parentheses. Histone modification profiles of the regions in young HSCs corresponding to Aged HSC open DARs are depicted in the right panels. b Chromatin accessibility (normalized ATAC counts) around Aged HSC open DARs in young and aged HSCs before and after stimulation with cytokines. c H3K27ac levels around Aged HSC open DARs in aged HSCs before and after stimulation with cytokines. d Box plots showing normalized ATAC counts (CPM) at Aged HSC open DARs (corresponding 37 promoters and 188 active, 91 primed, and 112 inactive enhancers) in young (left panels) and aged (right panels) HSCs before and after stimulation with cytokines. Data derived from a single experiment are depicted. Boxes represent the 25–75 percentile ranges. Horizontal bars represent medians. The ends of vertical lines represent minimum or maximum values. Paired Student’s two-tailed t tests were performed to calculate p values. e H3K27ac levels at Aged HSC open DARs (corresponding 37 promoters and 188 active, 91 primed, and 112 inactive enhancers) in aged HSCs before and after stimulation with cytokines. Data derived from a single experiment are depicted. ns, not significant. Paired Student’s one-tailed t tests were performed to calculate p values. Boxes represent the 25–75 percentile ranges. Horizontal bars represent medians. The ends of vertical lines represent minimum or maximum values. Source data are provided as a Source Data file.