Fig. 5: CSPG digestion reduces T cell infiltration and Th1 signature after spinal cord injury. | Nature Communications

Fig. 5: CSPG digestion reduces T cell infiltration and Th1 signature after spinal cord injury.

From: Chondroitin sulfate proteoglycans prevent immune cell phenotypic conversion and inflammation resolution via TLR4 in rodent models of spinal cord injury

Fig. 5

a Gating strategy used for lymphocyte recruitment assessment. b–d Graphs showing quantification of lymphocyte recruitment following SCI at b 7, c 14, and d 28 dpi with (LV-ChABC) or without (LV-GFP) CSPG digestion showing a significant reduction of T cell infiltration after CSPG digestion at 7 dpi. *p < 0.05, **p < 0.01 versus control (LV-GFP) group. Results were assessed for normality using the Shapiro–Wilk test and analysed using a two-tailed unpaired t test. Data are shown as mean ± SEM (n = 5 at 7 dpi, n = 6 at 14 dpi, and n = 3 at 28 dpi per treatment). Data are pooled from at least two independent experiments. e Th1 signature gene expression assessed by qPCR in TCD4 sorted cells. f IFNg expression comparison between LV-GFP and LV-ChABC treated animals in TCD4 sorted cells at 7 dpi. e, f *p < 0.05 versus control (LV-GFP) group. Results were assessed for normality using the Shapiro–Wilk test and analysed using a two-tailed unpaired t test. Data are shown as mean ± SEM (n = 4 per treatment). Detailed statistics and exact p values are provided in Supplementary Table 8. Source data are provided as a Source Data file.

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