Fig. 2: Adipocyte-specific IRAKM deficiency promotes energy expenditure.
a–c VO2 (a), VCO2 levels (b), and energy expenditure (EE) (c) of HFD-fed IRAKMF/F and IRAKMAKO mice were analyzed by Columbus Oxymax metabolic chambers. 12-h light/dark cycle; 72-h total duration; and each light/dark bar represents 12 h duration (n = 5 mice per group; from left to right: a P = 0.0031, 0.0014, 0.0014; b P = 0.032, 0.0013, 0.0008; c P = 0.0044, 0.0019, 0.0012). d Representative H&E staining of BAT sections from HFD-fed IRAKMF/F and IRAKMAKO mice (3 views per slide and 3 sections per mouse, n = 5 mice per group). e Thermogenic genes expression in BAT of HFD-fed IRAKMF/F and IRAKMAKO mice were analyzed by real-time PCR (n = 6; from left to right: P = 0.024, 0.035, 0.020, 0.038). f Rectal temperatures for HFD-fed IRAKMF/F and IRAKMAKO mice (n = 5; P = 0.033). g Body weight of IRAKMF/F and IRAKMAKO mice fed with zero-fat diet (ZFD) for 8 weeks (n = 6 males per group; P = 0.015). h EchoMRI analysis of IRAKMF/F and IRAKMAKO mice after ZFD feeding (n = 6; P = 0.011). i Representative H&E staining of BAT sections from ZFD-fed IRAKMF/F and IRAKMAKO mice (3 views per slide and 3 sections per mouse, n = 5 mice per group). j Thermogenic genes expression in BAT of ZFD-fed IRAKMF/F and IRAKMAKO mice were analyzed by real-time PCR (n = 6; from left to right: P = 0.011, 0.001, 0.005, 0.008). k Rectal temperatures for ZFD-fed IRAKMF/F and IRAKMAKO mice (n = 5; P = 0.019). l Western blot of Ucp1 in whole BAT tissue lysates from ZFD-fed mice. Data are representative of two independent experiments. Statistical significance was determined by two-way ANOVA (a–c) or two-tailed Student’s t-test (e–h, j, k). *P < 0.05. **P < 0.01. ***P < 0.001. All data represent mean ± s.e.m. Source data are provided as the Source data file.
