Fig. 3: IL-1β induces translocation of IRAKM into mitochondria to promote de novo fatty acid synthesis.

a, b Levels of selected fatty acids (more than 1 mg/g tissues) in iWAT (a) and BAT (b) from IRAKM^F/F and IRAKM^AKO mice after HFD feeding were detected with LC-MS/MS analysis (n = 3 mice per group; from left to right: a P = 0.022, 0.025, 0.016, 0.012, 0.03, 0.012, 0.006; b P = 0.039, 0.032, 0.011, 0.0019, 0.01). c De novo fatty acid synthesis measured by the conversion of ¹⁴C-glucose to lipids in primary adipocytes from WT and IRAKM KO mice treated with or without IL-1β for 24 h (n = 3; from left to right: P = 0.0026, 0.00025). d Mitochondrial (Mito) and cytosolic (Cyto) fraction were isolated from IL-1β treated primary adipocytes, followed by western blot analysis for indicated proteins. Data are representative of three independent experiments. e Western blot analysis of proteins of mitochondria (Mito), outer mitochondrial membrane (OM), inter mitochondrial membrane space (IMS), inner mitochondrial membrane (IM), and mitochondrial matrix (MA) of mitochondria from IL-1β treated primary adipocytes. Cyto C reductase, Cytochrome C reductase. Data are representative of two independent experiments. f OCR in newly differentiated primary WT and IRAKM KO adipocytes treated with or without IL-1β for 24 h (n = 4). g Maximum OCR in newly differentiated primary WT and IRAKM KO adipocytes treated with or without IL-1β for 24 h (n = 12 biologically independent samples; from left to right: P = 0.0006, 0.004). h, i Flag-tagged wild-type and IRAKM mito-mutant were restored in IRAKM KO primary adipocytes. h Mitochondrial (Mito) and cytosolic (Cyto) fraction were isolated from IL-1β treated primary adipocytes, followed by western blot analysis for indicated proteins. Data are representative of three independent experiments. i De novo fatty acid synthesis measured by the conversion of ¹⁴C-glucose to lipids in primary adipocytes treated with or without IL-1β for 24 h (n = 3; from left to right: P = 0.0005, 0.0005). Statistical significance was determined by two-tailed Student’s t-test (a–c, g, i). *P < 0.05. **P < 0.01. ***P < 0.001. All data represent mean ± s.e.m. Source data are provided as the Source data file.