Fig. 7: IRAKM kinase-dead KI mice are protected against obesity.
a Body weight of wide-type (WT) and IRAKM KI mice fed with high-fat diet (HFD) for 11 weeks (n = 7 males per group). b Insulin tolerance test (ITT) were analyzed in HFD-fed WT and IRAKM KI mice (n = 7 mice for each group; from left to right: P = 0.015, 0.013, 0.028, 0.0034, 0.011). c Representative H&E staining of iWAT sections from HFD-fed WT and IRAKM KI mice (3 views per slide and 3 sections per mouse, n = 5 mice per group). d VO2, VCO2 levels, and energy expenditure (EE) of HFD-fed WT and IRAKM KI mice were analyzed by Columbus Oxymax metabolic chambers. 12-h light/dark cycle; 72-h total duration; and each light/dark bar represents 12 h duration (n = 5 mice per group; from left to right: VO2: P = 0.012, 0.0075; EE: P = 0.016, 0.0085; VCO2: P = 0.029, 0.014). e Thermogenic genes expression in BAT of HFD-fed WT and IRAKM KI mice were analyzed by real-time PCR (n = 6; from left to right: P = 0.0012, 0.039, 0.017, 0.0012). f Representative H&E staining of BAT sections from HFD-fed WT and IRAKM KI mice (3 views per slide and 3 sections per mouse, n = 5 mice per group). g Body weight of WT and IRAKM KI mice fed with zero-fat diet (ZFD) for 8 weeks (n = 6 males per group; P = 0.046). h EchoMRI analysis of fat mass from WT and IRAKM KI mice after ZFD feeding (n = 6 males per group; P = 0.012). i Representative H&E staining of BAT sections from ZFD-fed WT and IRAKM KI mice (3 views per slide and 3 sections per mouse, n = 5 mice per group). j Thermogenic genes expression in BAT of ZFD-fed WT and IRAKM KI mice were analyzed by real-time PCR (n = 6; from left to right: P = 0.0004, 0.011, 0.037, 0.0016). k Western blot of Ucp1 in whole BAT tissue lysates from ZFD-fed mice. Data are representative of two independent experiments. Statistical significance was determined by two-way ANOVA (a, d) or two-tailed Student’s t-test (b, e, g, h, j). *P < 0.05. **P < 0.01. ***P < 0.001. All data represent mean ± s.e.m. Source data are provided as the Source data file.
