Fig. 5: Intracellular uptake of 1 wt% poly(CEP) induces global transcriptomic changes in RPE. | Nature Communications

Fig. 5: Intracellular uptake of 1 wt% poly(CEP) induces global transcriptomic changes in RPE.

From: A bio-functional polymer that prevents retinal scarring through modulation of NRF2 signalling pathway

Fig. 5

a Venn diagram showing overlap of differentially expressed genes (Wald test, Padj < 0.05) identified by DESeq2 at 24 h post-exposure to media only, poly(CEP), TNT or poly(CEP) + TNT. 1484 genes are common in both poly(CEP) and poly(CEP) + TNT treated RPE cells. b Gene set enrichment analysis (GSEA) was performed on the in vitro PVR model induced by TNT at 24 h post-exposure and showed upregulation of classical pathways (Hollow black stars) with known involvement in PVR pathogenesis. c GSEA was performed for 1 wt% poly(CEP) + TNT (PVR model) 24 h post-exposure and showed downregulation of EMT, migration, and cellular proliferation pathways, but upregulation of pathways involved in xenobiotics, proteosome degradation, and metabolism, similar to d 1 wt% poly(CEP) in media only. Hollow black stars mark unique pathways between (c, d).

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