Fig. 5: Interacting residues.

a, b Close-up of selected residues surrounding the bound 1PBC. c Concentration-response relations of 1PBC of selected mutants at a saturating Ca²⁺ concentration at −80 and 80 mV. Data are averages of the indicated number of biological replicates shown in Supplementary Table 1, errors are SEM. Solid lines are fits to the Hill equation. Dashed lines are the relations of WT. d Log-fold changes in IC₅₀ of mutants at 80 mV. Mutants of residues in contact with the blocker are shown in green. Bars indicate IC₅₀ values obtained via a fit of the averaged data shown in Supplementary Fig. 7 to the Hill equation, errors are 95% CI. The number of biological replicates is shown in Supplementary Table 1.