Fig. 2: R273 mutants orchestrate a distinct transcriptional signature.

a SW480 cells in which the endogenous TP53 genes (harboring R273H and P309S mutations) had been knocked out, were stably transduced with p53^R175H or p53^R273H. b Western blot analysis of p53 in SW480 knockout (KO) cells before and after transduction of p53^R175H or p53^R273H. n = 3. c SW480 TP53 KO cells and their derivatives expressing p53^R175H or p53^R273H were subjected to RNA-seq analysis. Shown is a heatmap of genes differentially expressed (fold change > 1.5, pAdj < 0.05) in p53^R273H overexpressing cells relative to p53 KO and p53^R175H overexpressing cells, n = 3. d Venn diagram of upregulated genes (fold change > 1.5, pAdj < 0.1) in p53^R273H overexpressors relative to p53 KO cells (blue circle) or p53^R175H overexpressors (green circle). The 140 overlapping genes were defined as the ‘R273 signature’. e Western blot analysis of p53 in SW480 cells stably transduced with shRNA directed against the 3’ UTR of the TP53 gene (shp53), followed by stable overexpression of shRNA-resistant p53^R175H or p53^R273H. shc = SW480 cells transduced with control shRNA, to visualize the endogenous p53. n = 3. f, g Gene Set Enrichment Analysis (GSEA) of differentially expressed genes in shp53 cells reconstituted with p53^R273H vs control shp53 cells or shp53 cells reconstituted with p53^R175H (ranked by fold change), using the R273 signature as the tested gene set. ES = Enrichment score. Source data is provided as a Source Data file.