Fig. 5: Characterisation of lycopene-producing strains with lycopene synthetic genes integrated at amplified RPL25 locus.
From: An in vivo gene amplification system for high level expression in Saccharomyces cerevisiae

a Schematic maps of genetic vectors used to introduce lycopene synthetic genes into yeast. b Lycopene production in flask cultivations. Yeast cells in exponential growth was inoculated into 20āml MES-buffered YNB medium with 20āgālā1 glucose in 125āml Erlenmeyer flask to start a culture at OD600ā=ā0.2. Mean valuesā±āstandard deviations are shown (Nā=ā4 independent biological replicates). Source data are provided as a Source Data file.