Fig. 5: Computer-assisted modeling aids with estimating resection cavity volume and the safety profile of EnMSCBif in vivo reveals no abnormalities in major organs. | Nature Communications

Fig. 5: Computer-assisted modeling aids with estimating resection cavity volume and the safety profile of EnMSCBif in vivo reveals no abnormalities in major organs.

From: RETRACTED ARTICLE: Target receptor identification and subsequent treatment of resected brain tumors with encapsulated and engineered allogeneic stem cells

Fig. 5

a Representative MR scan and computer modeling of tumor volume (green) and resection cavity volume (blue) using 3D slicer. b Plot representing tumor and resection cavity volumes obtained from retrospective analysis of ten deidentified GBM patients pre- and post-surgical debulking. c Illustration of the real-time formulation of GMP-MSCBif obtained from master cell bank post shipment. d Photograph of representative bags used for GMP-MSCBif shipment. e Plot showing the viability of MSCBif over time in 10%HSA. Data analyzed by comparing two groups using t test. f Plot showing gelation time of GMP-EnMSCBif with varying cell numbers. g Plot showing activation of HSV-TK kill switch in GMP-EnMSCBif following GCV administration. h Plot showing changes in tumor volume in mice bearing GBM tumors treated with varying doses of GMP-EnMSCBif post resection in a dose-escalation study. (n = 5 mice/ group). i Plot showing changes in weights of mice bearing GBM tumors treated with varying doses of GMP-EnMSCBif post resection over time. j Plot showing median gelation time of GMP-Hystem Hydrogel from the two different lots in the absence and presence of MSC. k Plot showing the viability of GBM cells following co-culture with GMP-EnMSCBif at specified time points. l Plot showing weights of mice (n = 5 mice/group) following administration of either GMP-Hystem hydrogel alone or GMP-EnMSCBif in NOD.SCID mice over time. m Photomicrographs of H&E stains of the mouse brain and other major organs following GMP-EnMSCBif administration in non-tumor-bearing mice. n Photomicrographs of brain tissues by expansion microscopy (dEXM) using GFAP and Nestin staining. Data shown for n = 3 biologically independent samples/dose (e–g). Statistical analyses were done using two-way ANOVA with multiple comparisons (h, k). Data are shown as mean ± S.E.M. Scale bars 200 μM (m), and 100 μM (n); **p < 0.05, ***p < 0.01.

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