Fig. 3: Effect of signal sequences on HsMOR function. | Nature Communications

Fig. 3: Effect of signal sequences on HsMOR function.

From: Functional expression of opioid receptors and other human GPCRs in yeast engineered to produce human sterols

Fig. 3

a N-terminal signal sequences tested on HsMOR. b Effect of signal sequences on HsMOR biosensor sensitivity to DAMGO and morphine. Unpaired one-way ANOVA of pEC50s: n = 3 biologically independent experiments, >7595 cells/strain/replicate; P < 0.0001 for each agonist; Dunnett’s multiple comparisons tests against WT shown. c Effect of signal sequences on HsMOR signaling population. Unpaired one-way ANOVA: n = 3 biologically independent experiments, >7595 cells/strain/replicate; P < 0.0001 (DAMGO) and P < 0.001 (morphine); Dunnett’s multiple comparisons tests against WT shown. d Imaging of HsMOR-GFP with the indicated signal sequences in a cholesterol-rich background with the ER marker RFP-SCS2(tmh). n = 3 biologically independent experiments. Data presented as mean +/− SEM. Scale bar is 2 μm. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001. Source data are provided as a Source Data file.

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