Fig. 4: The effects of HsMOR missense mutations on opioid biosensor activity. | Nature Communications

Fig. 4: The effects of HsMOR missense mutations on opioid biosensor activity.

From: Functional expression of opioid receptors and other human GPCRs in yeast engineered to produce human sterols

Fig. 4

a HsMOR snake plot with previously identified missense mutations. Biosensors based on these mutants were assayed for DAMGO response (b), or morphine response (c), by flow cytometry. Paired one-way ANOVAs of DAMGO pEC50s and maximum percentage of cells signaling: n = 6 biologically independent experiments, >2956 cells/condition/replicate; P = 0.0002 and P < 0.0001 respectively; Dunnett’s tests against WT shown. Paired one-way ANOVAs of morphine pEC50s and maximum percentage of cells signaling: n = 6 biologically independent experiments, >4761 cells/condition/replicate; P = 0.0033 and P < 0.0001 respectively; Dunnett’s tests against WT shown. Data presented as mean +/− SEM. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001. Source data are provided as a Source Data file.

Back to article page