Fig. 5: The functional requirements of HsMOR N- and C-terminal domains on biosensor activity. | Nature Communications

Fig. 5: The functional requirements of HsMOR N- and C-terminal domains on biosensor activity.

From: Functional expression of opioid receptors and other human GPCRs in yeast engineered to produce human sterols

Fig. 5

a Domains of Ste2, HsMOR, and HsMOR variants with the highly conserved seven transmembrane helix (7xTMH) domains indicated. b DAMGO and morphine sensitivity of biosensors based on the indicated HsMOR variants. Unpaired one-way ANOVA of pEC50s: n = 3 biologically independent experiments, >7464 cells/strain/replicate; P < 0.0001 for both agonists; Dunnett’s tests against WT shown. c The effect of N- and C-terminal HsMOR mutations on the population of signaling biosensor cells. Unpaired one-way ANOVA: n = 3 biologically independent experiments, >7464 cells/strain/replicate; P < 0.0001 for both agonists; Dunnett’s tests against WT shown. d Imaging of C-terminally GFP-tagged HsMOR variants in a cholesterol-producing background with ER marker RFP-SCS2(tmh). n = 3 biologically independent experiments. Data presented as mean +/− SEM. Scale bar is 2 μm. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001. Source data are provided as a Source Data file.

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