Fig. 6: The effect of membrane sterol composition on opioid biosensor signaling efficiency. | Nature Communications

Fig. 6: The effect of membrane sterol composition on opioid biosensor signaling efficiency.

From: Functional expression of opioid receptors and other human GPCRs in yeast engineered to produce human sterols

Fig. 6

a Construction of an array of putative biosensor strains with systematically varied promoter strength for the cholesterol biosynthetic genes downstream of zymosterol. Cassettes containing these genes under different promoters were integrated into an HsMOR biosensor background with an additional copy of these enzymes in place of the final ergosterol biosynthetic genes (ERG2/3/5/6). b Cholesterol biosynthesis intermediates after zymosterol. c Sterol analysis and full dose responses with DAMGO of 39% of biosensors from the collapsed screen. 10,000 cells/strain/replicate, n = 2, 3 or 4. Percentages of total sterol content for the intermediates were determined and mean pEC50s were calculated. Source data are provided as a Source Data file.

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