Fig. 3: In vitro screening of thiolase and termination enzymes to find specific variants for C4 and C6 products using the r-BOX core enzymes found in Fig. 2. | Nature Communications

Fig. 3: In vitro screening of thiolase and termination enzymes to find specific variants for C4 and C6 products using the r-BOX core enzymes found in Fig. 2.

From: Cell-free prototyping enables implementation of optimized reverse β-oxidation pathways in heterotrophic and autotrophic bacteria

Fig. 3

A Thioesterase and ptb-buk termination screen for the production of short-chain fatty acids. All assays contained each enzyme at 0.3 μM concentration. DJ52_buk was added to the enzymes containing ptb’s. B ACR-ADH and bifunctional AdhE screen for the production of butanol and hexanol. All assays contained thiolase and termination enzymes at 0.3 μM concentration and the three core enzymes Ck_hbd1, Ca_crt, and Td_ter at 0.15 μM due to volume limitations. Assays with an ACR were complemented with 0.15 μM of Aa_adh. All measured data points and their mean are shown with n greater than or equal to 3, and error bars represent standard deviation. Detailed results and full information on enzyme abbreviations in Source Data file.

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