Fig. 3: The PduB N-terminus links between the shell and the enzymatic core.
From: Biogenesis of a bacterial metabolosome for propanediol utilization
a Relevant cargo-encoding genes of relevance to Fig. 3b–d are highlighted in the context of the pdu operon of S. Typhimurium LT2. b PduE-sfGFP (green) and mCherry (red, labeling different catalytic components) were visualized in ΔpduB1–37 following growth in MIM in the absence and presence of 1,2-PD. c Colocalization analysis of sfGFP and mCherry fluorescence in (b). Note that in panels c, g, the first capital letter of the name refers to the Pdu protein tagged with mCherry and the second capital letter refers to the Pdu protein tagged with sfGFP. “+” and “−“ represent the presence and absence of 1,2-PD, respectively. Pearson’s R values for all the strains are: 0.68 ± 0.07 (G/E-); 0.53 ± 0.04 (L/E-); 0.01 ± 0.09 (O/E-); 0.28 ± 0.17 (P/E-); 0.70 ± 0.10 (Q/E-); 0.90 ± 0.08 (G/E+); 0.87 ± 0.07 (L/E+); 0.91 ± 0.08 (O/E+); 0.91 ± 0.05 (P/E+); 0.91 ± 0.10 (Q/E+). d The localization PduCDE and PduGH were characterized following growth in MIM-1,2-PD media. e Relevant genes encoding shell and catalytic proteins in f, g. f PduE-sfGFP (green) or PduA-sfGFP (green) and mCherry (red, labeling different structural components) were visualized in ΔpduB1–37, following growth in MIM + 1,2-PD media. g Colocalization analysis of sfGFP and mCherry fluorescence in (f). The Pearson’s R values for all the strains are: −0.05 ± 0.12 (B’/E); 0.72 ± 0.14 (B’/A); 0.05 ± 0.10 (J/E); 0.77 ± 0.12 (J/A); −0.05 ± 0.10 (K/E); 0.75 ± 0.08 (K/A); 0.84 ± 0.09 (M/E); 0.08 ± 0.10 (M/A). h Microscopic characterization of the ΔpduB1–37 strain following growth in MIM + 1,2-PD media, showing a polar enzymatic core and several empty shell structures (arrows). Note: In c, g, data are represented as mean ± SD. n = 20, n represents the number of cells. Boxplot center lines correspond to the median value, and upper and lower whiskers extend from the box to the largest or smallest value correspondingly, but no more than 1.5 times the interquartile range; mean values show as the square symbol.
