Fig. 5: Pdu BMCs undergo a combination of the “Shell first” and “Cargo first” assembly pathways.
From: Biogenesis of a bacterial metabolosome for propanediol utilization
a, b Aggregation of the shell (PduA-sfGFP or PduJ-mCherry) and cargo (PduE-sfGFP or PduE-mCherry) in the WT strain following induction with 1,2-PD (addition of 0.6% 1,2-PD to MIM media at t = 0). Red: protein labeled with mCherry; green: protein labeled with sfGFP. c The distribution of the number of “Shell first” and “Cargo first” events in PduE-mCherry/PduA-sfGFP and PduJ-mCherry/ PduE-sfGFP. d Progression of shell and cargo formation in WT strain with pBAD-pduJ-mCherry/pduE-sfGFP. Red: PduJ-mCherry; green: PduE-sfGFP. “Shell first” (white arrow, number 1) and “Cargo first” (yellow arrow, number 2) events co-occurred 1 h after addition of 1,2-PD to MIM media. Shell and cargo fluorescence colocalized at 1.5 h after the addition of 1,2-PD (white and yellow arrows). e Histogram of the spatial distribution of the first Pdu BMC along the long axis of the cell. n = 81, n represents the number of cells.
