Fig. 4: DMP1 secretion correlates positively with body weight and coincides spatiotemporally with FAM20C upregulation. | Nature Communications

Fig. 4: DMP1 secretion correlates positively with body weight and coincides spatiotemporally with FAM20C upregulation.

From: Mechanical forces couple bone matrix mineralization with inhibition of angiogenesis to limit adolescent bone growth

Fig. 4

a DMP1 and EMCN immunostaining of 5.5-week-old femurs derived from littermate mice. Red dotted line marks the area of extracellular DMP1 at the OF. Scale bar 250 µm. b Counterplot of body weight versus DMP1 intensity. R2 = 0.4139, n = 19. c Quantification of FAM20C intensity in the centre relative to periphery (centre + periphery) in 4- and 6-week-old femurs. Two-tailed paired t-test. d Quantification of FAM20C intensity in the centre in 4- and 6-week-old femurs. Two-tailed paired t-test. c, d Pooled data from two independent experiments. At 4 weeks n = 6, 6 weeks n = 5. c, d Source data are provided in Source Data file. e FAM20C and DMP1 immunostaining in OF of 4- and 6-week-old femurs. Scale bar 50 µm.

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