Fig. 2: Hypoxic PH is exacerbated in Pafah2 KO mice.

a Western blotting of PAF-AH2 in total protein from the lungs of WT mice and Pafah2 KO mice exposed to normoxia or hypoxia for 8 weeks (left) and quantification by densitometry (right). Experiments were repeated three times and the data were pooled. b The contents of ω-3 epoxides assessed by LC-MS/MS based-lipidomics of the lungs in WT mice and Pafah2 KO mice exposed to hypoxia for 8 weeks (n = 4,5). c Representative image of histological sections with HE staining (top) and EVG staining (bottom) of lungs in WT, Pafah2 KO, and Pla2g7 KO mice exposed to normoxia or hypoxia for 8 weeks. Scale bar, 50 μm. d–f The evaluation of PH severity in WT, Pafah2 KO, and Pla2g7 KO mice exposed to normoxia or hypoxia for 8 weeks (normoxia, n = 4,4,4; hypoxia, n = 6,8,6). Wall thickness of pulmonary arterioles (d), RVSP (e), weight ratio of RV to LV + septum (f). Experiments were repeated twice and the data were pooled (e, f). g Relative mRNA levels of Nppa in RV of hypoxia-exposed WT mice and Pafah2 KO mice (n = 6, 8). Expression levels were normalized to those of 18S ribosomal RNA and then to those in the RV of WT mice. h Kaplan–Meier curves of hypoxia-exposed WT, Pafah2 KO, and Pla2g7 KO mice until 100 days (n = 16). Log-rank test; WT vs Pafah2 KO, P = 0.002, Pafah2 KO vs Pla2g7 KO, P = 0.011. Experiments were repeated three times and the data were pooled. i Relative mRNA levels of Col1a1 in lungs of WT mice and Pafah2 KO mice exposed to normoxia or hypoxia for 8 weeks (n = 6). Expression levels were normalized to those of 18S ribosomal RNA and then to those in the lung of WT mice. j Representative image of Immunohistochemistry for Vimentin and CD31 in lungs of WT mice and Pafah2 KO mice exposed to hypoxia for 8 weeks. Nuclei were labeled with DAPI. Scale bar, 50 μm. Data are mean ± SEM. P values were determined by two-way ANOVA with Bonferroni’s post hoc test (a), one-way ANOVA with Tukey’s post hoc test (d–f), or 2-tailed Student’s T test (b, g, i).