Fig. 6: Pafah2 is a potent gene involved in the development of human PAH.
From: Omega-3 fatty acid epoxides produced by PAF-AH2 in mast cells regulate pulmonary vascular remodeling
a Schematic diagram of the locations of 2 pathogenic mutation candidates in human Pafah2 gene. b The structural model of PAF-AH2 p.R85C variant (yellow in left), p.Q184R variant (yellow in right) and native form (blue), showing the stacked snapshots of 300 frames obtained from frame 2700–3000 (corresponding to 54–60 nano-seconds). Arrows show the changed conformation in each variant model compared to native form model. c Western blotting of PAF-AH2 in HEK 293 cells expressing human Pafah2 variants using pcDNA vectors when treated with or without MG132 (10 μM) for 6 h (top), and quantification by densitometry (bottom). Experiments were repeated four times and the data were pooled. d Graphical summary. Data are mean ± SEM. P values were determined by two-way ANOVA with Tukey’s post hoc test.
