Fig. 1: An adaptive biomaterial based on self-assembled protein nanostructures on a liquid–liquid interface. | Nature Communications

Fig. 1: An adaptive biomaterial based on self-assembled protein nanostructures on a liquid–liquid interface.

From: Adaptive liquid interfaces induce neuronal differentiation of mesenchymal stem cells through lipid raft assembly

Fig. 1

a Schematic of neuronal differentiation of hMSCs at the interfacially assembled two-dimensional network of protein nanofibrils. b Fluorescence images of ThT-dyed lysozyme nanofibrils assembly at the PFO/water interface at pH 2 and pH 5. Scale bar: 100 μm. c Representative AFM images of lysozyme nanofibrils assembled at the PFO/water interface at pH 2 and pH 5. d Fluorescence images of FITC-lysozyme assembly at the PFO/water interface at pH 2 and pH 5. Scale bar: 100 μm. Inset shows the optical photograph of protein layer at the PFO/water interface under blue light irradiation. e Representative AFM images of protein nanosheets at the PFO/water interface at pH 2 and pH 5. f Time evolution of interfacial tension between lysozyme monomer/nanofibril aqueous solution and PFO at pH 5 and pH 2. g Images of the interfacial jamming process when PFO phase is withdrawn from the droplet in the bath of lysozyme nanofibrils solution at pH 5.

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