Fig. 2: Knockdown of NELF or CBP80 decreased the Pol II pausing at the TES proximal region of RDH genes. | Nature Communications

Fig. 2: Knockdown of NELF or CBP80 decreased the Pol II pausing at the TES proximal region of RDH genes.

From: The 3′ Pol II pausing at replication-dependent histone genes is regulated by Mediator through Cajal bodies’ association with histone locus bodies

Fig. 2

a, b Genome browser tracks showing the distribution of PRO-seq reads at HIST1H1E (a) and HIST1H2AL (b) in control, NELF-knockdown (NELF-E si) and CBP80-knockdown (CBP80 si) cells. The positive and negative strands of DNA are indicated by (+) and (−), respectively. c, d Meta-gene analysis of PRO-seq reads around TESs of RDH genes (c) and snRNA genes (d) in control, NELF-knockdown (NELF-E si) and CBP80-knockdown (CBP80 si) cells. e Boxplots representing the TPP index for RDH genes (n = 33), snRNA genes (n = 25), snoRNA genes (n = 80) and rRNA genes (n = 8) in control, NELF-knockdown (NELF-E si) and CBP80-knockdown (CBP80 si) cells. The center line of each boxplot represents the median. Upper and lower fences of each boxplot represent upper and lower quartiles, respectively. The P-values were determined by two-sided Wilcoxon’s signed-rank test (*P < 0.05; **P < 0.01). N.S., not significant. Source data and exact P-values are provided as a Source Data file.

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