Fig. 1: Proteomic analysis and GO term enrichment analysis of candidate MPS-interacting proteins.

a Schematic for co-immunoprecipitation (co-IP)-based mass spectrometry identification of candidate MPS-interacting proteins. The antibody against a bait protein, βII-spectrin, αII-spectrin, or α-adducin, was attached to protein-G-coated beads. The antibody-coated beads were then used to capture the bait protein and the co-immunoprecipitated proteins from the cultured hippocampal neuron lysate or adult mouse whole-brain lysate. The co-immunoprecipitated proteins were identified using mass spectrometry. b Top: Venn diagrams showing the overlap of the identified proteins in three co-IP experiments using βII-spectrin, αII-spectrin or α-adducin as the bait, either from cultured hippocampal neuron (DIV 20) lysates (left) or from mouse whole-brain lysates (right). Two biological replicates were performed for each co-IP condition. Bottom: Venn diagram showing the overlap between the 480 identified proteins from cultured hippocampal neurons and the 670 identified proteins from mouse whole-brain lysates (See Supplementary Data 1 for the full list of the candidate MPS-interacting proteins). c Functional annotation clustering of the enriched Gene Ontology (GO) terms in the biological process (BP) category for the 480 candidate MPS-interacting proteins identified in cultured hippocampal neurons. A selected subset of GO BP terms are shown (See Supplementary Data 2 for the full list of clustered GO BP terms enriched in the candidate MPS-interacting proteins). The corresponding p-values (bars) and protein numbers (next to the bars) for each enriched GO BP term are shown on the right. d Venn diagrams showing the overlap between the enriched GO BP terms of the 480 candidate MPS-interacting proteins identified in cultured mouse hippocampal neurons (DIV 20) and the enriched GO BP terms of the 670 candidate MPS-interacting proteins identified in the adult mouse whole brain (left). Also shown in comparison is the overlap between the enriched GO BP terms of the 480 candidate MPS-interacting proteins in cultured mouse hippocampal neurons (DIV 20) and the enriched GO BP terms of 670 randomly selected genes from the mouse genome (right). e Same as d but for GO MF terms instead of GO BP terms.