Fig. 2: RDO5 promotes antiviral RNAi.

a–h Accumulation of CMV-∆2b (a, b, e, f) or CMV-2aT∆2b (a, d, g, h) detected in wild-type (Col-0), single, double or triple mutant plants as indicated at 2 weeks post-infection by ELISA (a, e, g) and Western blot analysis of the viral coat protein (CP) or Northern blot analysis of the viral RNAs 1-4 (b, d, f, h). Detection of the vsiRNAs and plant endogenous small RNAs (b, d), and the calculation of vsiRNAs/gRNAs ratios (c) were as described in the legend to Fig. 1. Note the reduced sample loading (to ½) for total proteins and total RNAs (f) from ago1/2 double mutant plants and rdo5/ago1/2 triple mutant plants infected with CMV-∆2b. Data presented are means ± SEM from three replicates (a, e, g) or independent experiments (c), letters indicate groups with significant differences (one-way ANOVA, Duncan, p < 0.05) and black dots represent the individual values. The experiments in (b), (d), (f) and (h) were repeated three times independently with similar results. The source data underlying blots in (b), (d), (f) and (h), ELISA data in (a), (e) and (g), and ratio data in (c) are provided as a Source Data file.