Fig. 3: Identification of VIR1.
a Manhattan plot for GWAS mapping of the susceptibility phenotype to Q-CMV infection. The horizontal dash-dot line corresponds to the significance threshold (p = 5.45 × 10−7). The black tringle above the threshold indicates the most significantly associated locus. b Regional Manhattan plot (from 1512 kb to 1522 kb), structure of VIR1, and positions of T-DNA insertion allele vir1-1 and CRISPR/Cas9 genome edited alleles (cr5 in At5g05130 and cr6-14 and cr6-31 in VIR1). c, d, f, Accumulation of Q-CMV (c, d) or Q-CMV-∆2b (c, f) detected in wild-type (Col-0), cr5 mutant, vir1 mutants (vir1-1, cr6-14 and cr6-31), or two independent VIR1-complemented lines of vir1-1 at 2 weeks post-infection by RT-qPCR analysis of the viral RNA3 (c) or Northern blotting of the viral RNAs 1-4 (d, f). The vsiRNAs and plant endogenous small RNAs were also detected by Northern blotting (d, f). e The vsiRNAs/gRNAs ratios were calculated as described in the legend to Fig. 1. Data in (c) and (e) are means ± SEM from three independent experiments, letters indicate groups with significant differences (one-way ANOVA, Duncan, p < 0.05) and black dots represent the individual values. The source data underlying blots in (d) and (f), qRT-PCR data in (c) and ratio data in (e) are provided as a Source Data file.
