Fig. 6: Summary of the derivation and characterization of hTSL cells.

Both alleles of the C19MC DMR are hypomethylated in naïve hES and hTSL^naïve cells, but hypermethylated in primed hES and hTSL^primed cells. The C19MC DMR is demethylated in hTSL^C19MC and hTSL^primed-C19MC cells using the dCas-TET system. Although hTSL^naïve and hTSL^C19MC cells are highly proliferative and can differentiate into both EVT- and ST-like cells, hTSL^primed and hTSL^primed-C19MC cells have poor proliferation and differentiation potentials. The C19MC DMR is highlighted in yellow. Black and white circles indicate methylated and unmethylated CpGs, respectively. M: maternal allele, P: paternal allele.