Fig. 3: Detailed map of the plasmids cr3Ec, pSwap and pDrop. | Nature Communications

Fig. 3: Detailed map of the plasmids cr3Ec, pSwap and pDrop.

From: A multifunctional system for genome editing and large-scale interspecies gene transfer

Fig. 3

The outer layer contains relevant features of each plasmid. The white inner layer (if applicable) represents the modular parts prior to assembly using MoCloFlex (MCF) or the CRISPR SWAPnDROP assembly system. The black inner layer (if applicable) shows the location of variable elements (orange) of the plasmids specific for each edit. Cas9 and meganuclease target sites are indicated by scissors. The sgRNA loci and the respective target sites share the same colour.

Back to article page